Glycosylation of Homologous Immunodominant Proteins of Ehrlichia chaffeensis and Ehrlichia canis
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منابع مشابه
Evaluation of antibiotic susceptibilities of Ehrlichia canis, Ehrlichia chaffeensis, and Anaplasma phagocytophilum by real-time PCR.
We determined MICs of antibiotics against Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Ehrlichia canis by real-time quantitative PCR. The doubling times of the organisms were established: 19 h for E. chaffeensis, 26 h for A. phagocytophilum, and 28 h for E. canis. In comparison to the reference method for determining sensitivities, which uses Diff-Quick staining, our PCR assay was very...
متن کاملSurface-exposed proteins of Ehrlichia chaffeensis.
The surface proteins of Ehrlichia chaffeensis provide an important interface for pathogen-host interactions. To investigate the surface proteins of E. chaffeensis, membrane-impermeable, cleavable Sulfo-NHS-SS-Biotin was used to label intact bacteria. The biotinylated bacterial surface proteins were isolated by streptavidin-agarose affinity purification. The affinity-captured proteins were separ...
متن کاملUltrastructure of Ehrlichia canis.
The ultrastructure of Ehrlichia canis was examined in both pulmonary mononuclear cells and in monocytes cultured from an infected dog. The cytoplasmic inclusions, or morulae, of E. canis consisted of a membrane-lined vacuole-containing elementary bodies which varied in size and number. The elementary bodies were bound by two trilamellar membranes. The organism shared morphological properties of...
متن کاملCharacterization of the major antigenic protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis.
Canine monocytic ehrlichiosis, caused by Ehrlichia canis or Ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. Coinfections with these agents may be common in certain areas of endemicity. Currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. Previously, we developed two indirect enzyme-linked immunosorbent as...
متن کاملEhrlichia chaffeensis in Child, Venezuela
To the Editor: Human mono-cytic ehrlichiosis is a tick-borne infectious disease caused by Ehrlichia chaffeensis (1). Serologic studies have indicated E. chaffeensis infection in Latin American countries: Venezuela (2), Mexico (3), Argentina (4), Chile (5), and Brazil (6). However, no molecular evidence for E. chaffeensis has been reported. In December 2001, a 9-year-old boy was admitted to a ho...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2000
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.68.1.13-18.2000